internal standards c17 ceramide  (Croda International Plc)

Journal: Nutrients

Article Title: Sulforaphane Attenuates Nonalcoholic Fatty Liver Disease by Inhibiting Hepatic Steatosis and Apoptosis

doi: 10.3390/nu14010076

Figure Lengend Snippet: Effects of SFA on alleviating PA-induced apoptosis in HepG2 cells: ( A ) apoptosis detected by annexin V (FITC)/PI staining and the percentage of apoptotic cells was calculated; ( B ) Western blot analysis showed the expressions of cleaved caspase 3 and gray analysis of each band; ( C ) ROS levels evaluated by DCFH-DA staining and expressed as mean fluorescence intensity relative to that in control group; ( D ) total ceramide contents detected by LC/MS/MS. All values are expressed as means ± SD from three independent determinations. * p < 0.05 vs. control cells; # p < 0.05 vs. PA-treated cells.

Article Snippet: Then, quantification of ceramides was performed on an Agilent high-performance liquid chromatography (HPLC) system, coupled with a quadrupole-time of flight mass spectrometer (6545 Q-TOF), as described previously [ , ], based on the C17-ceramide internal standards from Avanti Polar Lipids (Alabaster, AL, USA), as well as C14-ceramide, C18-ceramide, C20-ceramide, C22-ceramide, C24-ceramide, C24:1-ceramide, and C26:1-ceramide standards from ZZStandard (Shanghai, China).

Techniques: Staining, Western Blot, Fluorescence, Liquid Chromatography with Mass Spectroscopy

Journal: Nutrients

Article Title: Sulforaphane Attenuates Nonalcoholic Fatty Liver Disease by Inhibiting Hepatic Steatosis and Apoptosis

doi: 10.3390/nu14010076

Figure Lengend Snippet: Evaluation of hepatic apoptosis and ceramide contents after SFA treatment to HFD-fed C57BL/6J mice: ( A ) the hepatic apoptosis in different groups examined by TUNEL assay. ( n = 5/group); ( B ) Western blot analysis showed the levels of cleaved caspase 3 and ( C ) gray analysis of each band in liver ( n = 3/group); ( D ) total ceramide contents in liver detected by LC/MS/MS ( n = 4–5/group). All values are expressed as means ± SD, * p < 0.05 vs. NC group; # p < 0.05 vs. HFD group.

Article Snippet: Then, quantification of ceramides was performed on an Agilent high-performance liquid chromatography (HPLC) system, coupled with a quadrupole-time of flight mass spectrometer (6545 Q-TOF), as described previously [ , ], based on the C17-ceramide internal standards from Avanti Polar Lipids (Alabaster, AL, USA), as well as C14-ceramide, C18-ceramide, C20-ceramide, C22-ceramide, C24-ceramide, C24:1-ceramide, and C26:1-ceramide standards from ZZStandard (Shanghai, China).

Techniques: TUNEL Assay, Western Blot, Liquid Chromatography with Mass Spectroscopy

Journal: EBioMedicine

Article Title: A circular network of coregulated sphingolipids dictates lung cancer growth and progression

doi: 10.1016/j.ebiom.2021.103301

Figure Lengend Snippet: Serum sphingolipidomics analysis indicates altered sphingolipid metabolic states in NSCLC patients as compared with healthy individuals. ( a ) Schematic representation of the workflow for sample allocation as well as the extraction and identification procedure of serum sphingolipids from NSCLC patients and healthy individuals. ( b, c ) Manhattan plots reveal the relative levels of non-clinically relevant sphingolipid subclasses, including ceramide (Cer), ceramide-1-phosphate (C1P), sphingosine (SPH), sphingosine-1-phosphate (S1P), sphingomyelin (SM) and the relative levels of clinically relevant sphingolipid subclasses, such as galactosylceramide (GalCer)/glucosylceramide (GlcCer), sulfatide, lactosylceramide (LacCer), lacto/neolacto-series glycosphingolipid (lacto/neolacto-series GSL) in NSCLC patients as compared to healthy individuals. Values are shown as log 2 fold-change relative to healthy individuals. Each dot represents a sphingolipid species, color codes per sphingolipid subclass. Dot size indicates significance (-log 10 value). Serum sphingolipidomics data are combined of 100 NSCLC patients and 100 healthy individuals from the training dataset and represented as mean. ( d ) Analysis of correlations between different sphingolipid subclasses, as function of correlation strength. Black dotted line represents strongly correlated sphingolipids. ( e ) Overview of different sphingolipid levels in NSCLC patients. lacto/neolacto-series glycosphingolipid and sulfatide species are indicated in a close-up of the sphingolipid metabolic network. Red color represents up-regulated sphingolipids and green for down-regulated sphingolipids. Blue dotted line represents p < 0.05. All data are obtained from the training dataset. ( b, c ) Student's t test.

Article Snippet: Briefly, 100 μl serum of NSCLC patient or healty individuals was accurately weighed into a glass bottle, in which 1.5 ml of chloroform (CHCl 3 ) /MeOH (1:2, v/v) and internal standard C17 Ceramide (d18:1/17:0) (Cat# 860517, Avanti Polar Lipids), sphinganine-D7 (Cat# 860658, Avanti Polar Lipids) and sphingosine1-phosphate-D7 (Cat# 860641, Avanti Polar Lipids) was added.

Techniques: Extraction

Journal: Journal of the American Society for Mass Spectrometry

Article Title: A Novel Rapid MALDI-TOF-MS-Based Method for Measuring Urinary Globotriaosylceramide in Fabry Patients

doi: 10.1007/s13361-015-1318-4

Figure Lengend Snippet: MALDI-TOF/TOF MS/MS of ( a ) Gb3-Internal standard m/z 1060.7 (C17:0); and the three most abundant Gb3 species in Fabry patient urine: m/z 1130.7 (C22:0), 1158.7 (C24:0), and 1174.7 (C24:0-OH). The peaks corresponding to ions generated by the loss of 1 or 2 sugars (–162 and –324 Da, respectively) have different m/z values for each species, whereas the fragments corresponding to sphingosine –2H 2 0, ( m/z 264.1) and di- and tri-saccharide ions ( m/z 347 and 509) are invariant

Article Snippet: Porcine Gb3 standard and N -heptadecanoyl ceramide trihexoside (C17:0) internal standard were purchased from Matreya (Pleasant Gap, PA, USA).

Techniques: Tandem Mass Spectroscopy, Generated